Occasionally cell lines will become difficult and demonstrate poor growth. Our technical team has developed several proprietary methods for improving cell viability when these problems appear.
Occasionally cell lines may exhibit a degree of clonal instability which will result in a reduction in expression levels and, if allowed to continue, a total loss of secretion. Providing the original antigen is available for use in an ELISA assay it is possible to re-clone hybridoma lines to improve performance. If clonality is retrievable cells will be banked and then supplied to the customer.