Monoclonal antibody projects are customized according to the client needs. The project can start or finish at any phase and any moment and no project will continue to the next phase without written confirmation being received from the client. APS offers a range of extra options and all depends on needs of the customer. APS can offer services in mice (usually 4 BALB/c) or rat. APS has standard immunisation protocols which can be adapted to the client needs and discussed with the technical team.
Phase 0 - (3-4 weeks) Antigen design, antigen production and antigen conjugation.
Phase 1 - (up to 12 weeks) Immunisation of four mice, collection of four test bleeds and determination of ELISA titres.
Phase 2 - (3 weeks) Fusion of B-cells and myeloma and screening.
Phase 3 - (2 weeks) Primary subcloning of “hit(s) of interest” (one round) to ensure stability.
Phase 4 - (4 weeks) Preparation of hybridoma for client/scale up (includes extra subcloning and cell banking ampoules stock).
Phase 5 - (4-6 weeks) Scale up of hybridoma with option for serum free media.
Phase 6 - (2 weeks) Purification, dialysis and characterisation of antibody.